By Baldassare Di Bartolo; Ottavio Forte; International School of Atomic and Molecular Spectroscopy.; North Atlantic Treaty Organization
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Mikes, Y. Yang, I. Teraoka, T. Ishigure, Y. Koike, and Y. Okamoto,”Synthesis and Characterization of an Amorphous Perfluropolymer: Poly(perfluoro-2methylene-4-methyl-1,3-dioxolane”, Macromolecules 38, 4237-4245(2005). 21. O. Gaathon, W. Jeck, I. Teraoka, and S. Arnold (in progress). it, Abstract The first laser has been invented in 1960, and since then the laser field exploded almost exponentially, covering a spectral range from less than 1 Å to more than 1 mm. Many of them have been used with outstanding results both in basic science, and in industrial and commercial applications, by changing for ever the same lifestyle of humankind.
With this restricted dimension Eqn. 2 can be reduced to an approximate form 2 δλ t § δ (n ) · = ¨¨ 2 2 ¸¸ λ a © ns − nm ¹ (6) Eqn. δλ/λ). We see from Eqn. 4 that this normalized shift is simply proportional to the thickness of the layer. This suggests that protein with differing molecular weight may give different normalized shifts. Based on this reasoning we carried out a number of experiments in which we adsorbed protein molecules with molecular weights ranging over more than two orders of magnitude.
7 Resonance wavelength shift vs. MW. Form lowest to highest: Insulin, Į-Lactalbumin, BSA, γγ-Globulin, and Thyroglobulin. The line was drawn manually to fit the data points. 14 be nearly constant at saturation. Under these circumstances t dominates the shift. With the protein volume proportional to the molecular weight, the thickness would be expected to be proportional to the cube root of the molecular wt. The small scatter of the points around the MW1/3 dependence is surprising considering that protein have different shapes from one to another.
Advances in spectroscopy for lasers and sensing by Baldassare Di Bartolo; Ottavio Forte; International School of Atomic and Molecular Spectroscopy.; North Atlantic Treaty Organization