Ute Resch-Genger, Markus Grabolle, Roland Nitschke, Thomas's Advanced Fluorescence Reporters in Chemistry and Biology II: PDF

By Ute Resch-Genger, Markus Grabolle, Roland Nitschke, Thomas Nann (auth.), Alexander P. Demchenko (eds.)

ISBN-10: 3642046991

ISBN-13: 9783642046995

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“This booklet is a finished review of the different sorts of nanoscale probes which were and are at the moment being constructed for sensing of analytes. The well timed reference part permits the reader to find key references to the first literature for enlargement of the cloth lined. … a necessary source that covers the factitious, photo-physical, and theoretical facets of this diversified box of research.” (Jeffrey T. Petty, magazine of the yankee Chemical Society, Vol. 133, 2011)

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Additional resources for Advanced Fluorescence Reporters in Chemistry and Biology II: Molecular Constructions, Polymers and Nanoparticles

Example text

Zhang J, Campbell RE, Ting AY, Tsien RY (2002) Creating new fluorescent probes for cell biology. Nat Rev 3:906–918 4. Lakowicz JR (2006) Principles of fluorescence spectroscopy, 3rd edn. Springer, New York 5. Grabolle M, Kapusta P, Nann T, Shu X, Ziegler J, Resch-Genger U (2009) Fluorescence lifetime multiplexing with nanocrystals and organic labels. Anal Chem 81:7807–7813 6. Sapsford KE, Berti L, Medintz IL (2006) Materials for fluorescence resonance energy transfer analysis: beyond traditional donor–acceptor combinations.

A superior long-term stability compared to organic dyes has been demonstrated for example for CdSe/ZnS and rhodamine-labeled tubulin [42] CdSe and Texas Red [81] as well as for antibodies labeled with CdSe, FITC, R-phycoerythrin, and AlexaFluor 488 [77]. However, nanoparticles can show specific phenomena such as photobrightening [82] see also Sect. 7 on Reproducibility, Quality Assurance, and Limitations, and undesired aggregation of nanocrystals can contribute to reduced stability. The thermal and photochemical stability of both organic dyes and nanocrystals are influenced by an extremely broad variety of conditions that need to be considered: excitation wavelength and intensity, matrix or microenvironment, label concentration, and, in the case of nanoparticles, surface chemistry.

Sackett DL, Wolff J (1987) Nile red as a polarity-sensitive fluorescent probe of hydrophobic protein surfaces. Anal Biochem 167:228–234 30. 5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalently linking to IgC and noncovalent binding to avidin. Bioconjug Chem 11:696–704 31. Soper SA, Mattingly QL (1994) Steady-state and picosecond laser fluorescence studies of nonradiative pathways in tricarbocyanine dyes: implications to the design of near-IR fluorochromes with high fluorescence efficiencies.

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Advanced Fluorescence Reporters in Chemistry and Biology II: Molecular Constructions, Polymers and Nanoparticles by Ute Resch-Genger, Markus Grabolle, Roland Nitschke, Thomas Nann (auth.), Alexander P. Demchenko (eds.)


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